Object Aperture: 13 pixels                                                    
                                                                              
Inner Annulus: 25 pixels                                                      
                                                                              
Outer Annulus: 60 pixels                                                      
                                                                              
                                                                              
Frame 107: Guide 1 drops off chip                                             
                                                                              
Frame 125: Guide 2 drops off chip                                             
                                                                              
Frame 139: Guide 3 drops off chip